GPx1 gene over-expressed or knocked-down each group of indicators
Published: 2 August 2018| Version 1 | DOI: 10.17632/y5245j5jrg.1
Contributor:
昌豪 陈Description
This data is the detection result of each indicator of GPx1 gene overexpression or knockdown spleen lymphocytes. These include antioxidant markers, apoptotic genes, and methylation-related genes. ELISA and RT-PCR were used for detection.
Files
Steps to reproduce
ELISA kits (ELISA kit is purchased from Nanjing Jiancheng Bioengineering Institute, China) were employed to determine the antioxidant related indexes in the cell supernatant or precipitant. The absorbance at 405, 450 and 532 nm was measured using a spectrophotometer. The results were calculated on the excel table according to the formula on the manual, and the paired T test was performed at the end with SPSS 22.0 software.
Institutions
Sichuan Agricultural University
Categories
Polymerase Chain Reaction, Enzyme-Linked Immunosorbent Assay