Engineered Lactobacillus paracasei 3×pBD1/ΔHLJ-27 as a Novel Antibiotic Alternative for Livestock: Dual Efficacy in Growth Promotion and Protection Against Salmonella Infection via Integrated Host-Microbiota-Immune Regulation
Description
Fig.1 3×pBD1/ΔHLJ-27 colonizes the intestine to improve growth and barrier integrity in BALB/c mice and piglets. Fig.2 Systemic and mucosal immune enhancement by 3×pBD1/ΔHLJ-27. Detection of CD4⁺ T cell content in mesenteric lymph nodes of mice. Fig.3 Efficacy of oral recombinant strain against intestinal S. Typhimurium infection in mice. Fig.4 Recombinant strain improves intestinal barrier function and modulates immune responses during S. Typhimurium infection in mice. Fig.5 Protective efficacy and bacterial clearance in S. Typhimurium-infected piglets following administration with the recombinant strain 3×pBD1/ΔHLJ-27 in piglets. Fig.6 3×pBD1/ΔHLJ-27 protects intestinal morphology and mucus barrier during S.Typhimurium infection in piglets. Fig.7 3×pBD1/ΔHLJ-27 restores intestinal barrier integrity by regulating tight junction proteins following Salmonella infection in piglets. Fig.8 Recombinant strain 3×pBD1/ΔHLJ-27 confers systemic protection against S. typhimurium infection by orchestrating integrated immune and antioxidant responses in piglets. Fig.9 Effects of 3×pBD1/ΔHLJ-27 on intestinal microflora and ileum tissue in piglets. S1 Fig. 3×pBD1/ΔHLJ-27 colonizes the intestine to improve growth and barrier integrity in BALB/c mice and piglets. S2 Fig. The changes of intestinal microbial diversities in piglets with oral administration of 3×pBD1/ΔHLJ-27.