Open Reading Frames (ORFs) and annotations from the paired-end transcriptome outputs of Savalia savaglia's RNASeq data
Description
This dataset contains Open Reading Frames (ORFs) and annotations obtained from the paired-end transcriptome outputs of Savalia savaglia's RNASeq data. The dataset includes the following files: From TransDecoder analyses: • Assembly_Ss_PE.Trinity.fasta.transdecoder.cds: Nucleotide sequences for coding regions of the final candidate ORFs, obtained with TransDecoder from paired-end transcriptome outputs of Savalia savaglia's RNA-Seq. • Assembly_Ss_PE.Trinity.fasta.transdecoder.gff3: Positions within the target transcripts of the final selected ORFs, obtained with TransDecoder from paired-end transcriptome outputs of Savalia savaglia's RNA-Seq. • Assembly_Ss_PE.Trinity.fasta.transdecoder.pep: Peptide sequences for the final candidate ORFs, obtained with TransDecoder from paired-end transcriptome outputs of Savalia savaglia's RNA-Seq. • Assembly_Ss_PE.Trinity.fasta.transdecoder.bed: BED-formatted file describing ORF positions, suitable for viewing using GenomeView or IGV, obtained from paired-end transcriptome outputs of Savalia savaglia's RNA-Seq data. • blastp.outfmt6.w_pct_hit_length: File providing percentages of hit lengths from BLASTp results of the paired-end de novo assembly of Savalia savaglia. It includes the top hit's length and the percentage of the length covered in the alignment. • pfam.domtblout: PFAM domain annotations for the predicted proteins in the paired-end de novo assembly of Savalia savaglia. From Trinotate analyses: • myTrinotate_PE_Ss.tsv: Comprehensive annotation file with results from Trinotate, including protein domain identification and other annotations from the paired-end de novo assembly of Savalia savaglia. • Trinotate_PE_Ss_report.cXp_summary.html: HTML report summarizing the annotation results from Trinotate, providing an overview of the functional annotations and transcript features of the paired-end de novo assembly of Savalia savaglia.
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Steps to reproduce
ORFs and annotation were generated by TransDecoder v5.7.1 and Trinotate v4.0.2 using the paired-end de novo assembly of the false coral Savalia savaglia.
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Funding
This work was supported by Centro Ricerche ed Infrastrutture Marine Avanzate in Calabria (CRIMAC) - Fondo FSC 2014-2020 - Piano Stralcio «Ricerca e Innovazione 2015-2017» – Programma Nazionale Infrastrutture di Ricerca (PNIR), CUP C64I20000320001.