Data for: Hormonal and Morphological Responses of A. platensis to Organic and Inorganic Carbon Sources in Monoculture and Consortium

Description

This dataset contains the experimental results and observations supporting the research on the physiological plasticity of Arthrospira platensis UTEX LB 1926. The data provides a comprehensive characterisation of microalgal performance when grown as a monoculture (acclimatised to organic environments) and in consortium with Pseudoanabaena sp. under three distinct cultivation regimes: inorganic (M1), organic-mixotrophic with lactic acid (M2), and a seawater-organic mixed medium (M3).

Steps to reproduce

The data were generated through a structured 8-day experimental period evaluating the physiological and morphological performance of Arthrospira platensis UTEX LB 1926. Two distinct biological configurations were compared: a monoculture (SA) previously acclimated to dairy wastewater (representing a pre-conditioned system for organic-rich environments) and a stable consortium (SC) with Pseudoanabaena sp. The experimental design followed a 2x3 factorial approach (two biological systems cultivated in three distinct media), resulting in six experimental treatments performed in independent triplicates. Three application-relevant media were formulated: M1 (Inorganic): A modified UTEX Spirulina medium (omitting vitamin B12) serving as a photoautotrophic reference with high inorganic carbon availability (NaHCO₃/Na₂CO₃). M2 (Organic): A synthetic medium where inorganic carbon was replaced by 2.5 g L⁻¹ of lactic acid to simulate mixotrophic growth in wastewater-like environments. M3 (Seawater-Organic): A complex system using ultrafiltered natural seawater (RIOKA®, Spain) as a base, supplemented with 0.25 g L⁻¹ of lactic acid and basic nitrogen/phosphorus (NaNO₃, K₂HPO₄) All media were adjusted to an initial pH of 9.5 and sterilized Cultivation was conducted in a Conviron® SH10 growth chamber at 25 °C, with a 12/12 h photoperiod and a photon fluency rate of 150 μE m⁻² s⁻¹ under 120 rpm agitation. Data Collection Protocols Biomass and Physicochemical Monitoring: Total dry biomass (g L⁻¹), pH, and electrical conductivity (EC) were recorded on days 0, 2, 4, 6, and 8. Biomass was determined gravimetrically by filtering 50 mL of homogenized culture through 0.45 µm membranes and oven-drying at 40 °C for 48 hours until constant weight Biochemical Analysis: Chlorophyll-α was extracted from fresh biomass using 80% (v/v) acetone and quantified via UV-Vis spectroscopy (Shimadzu UV-2450) at 660 nm and 645 nm. Phytohormone Profiling: Endogenous levels of IAA, JA, and PAA were determined using an LC–ESI(–)–MS/MS system (Agilent 1100 HPLC coupled with an API 3000 triple quadrupole mass spectrometer). Extraction involved a methanol:isopropanol:glacial acetic acid (20:79:1) mixture, with internal deuterated standards (d5IAA, d5JA, d5PAA) used for precise quantification Morphological Analysis: Filament length (µm), width (µm), and spiral degree (°) were captured using a Nikon Japan OPTIPHOT-2 microscope at 4X and 10X magnification Raw images were processed using MoranaS, a custom image analysis software specifically designed to automate the measurement of cyanobacterial filaments.

Categories

Funders

• Universitat Autònoma de Barcelona

  Cerdanyola del Vallès
• Universidad de Medellín

  Medellín
• Universidad EAFIT

  Medellín

Licence