Sequencing data of mouse ovarian tissue after heat stress treatment
Description
This document presents the sequencing data and analysis of mouse ovarian tissue following heat stress (HS) treatment. A systematic evaluation of gene expression levels was conducted on samples from the mouse ovarian HS treatment group and the Control group. First, quality control was performed on the sequencing data, and Clean reads were aligned to the mouse reference genome using STAR. Transcript quantification was then performed via StringTie to obtain the FPKM (Fragments Per Kilobase of transcript per Million mapped reads) values and corresponding raw counts for each gene, which were used for subsequent analysis. GO enrichment analysis showed that differentially expressed genes were significantly enriched across three levels: Biological Process (BP), Cellular Component (CC), and Molecular Function (MF). KEGG pathway enrichment analysis further revealed that HS treatment significantly affected multiple functional pathways. Among them, the Motor proteins pathway (mmu04814) was significantly enriched, and the enriched genes mainly include multiple members of the dynein and kinesin families, which is highly consistent with the cilia- and microtubule-related functions indicated in the GO analysis. GSEA-GO enrichment results demonstrated that in the HS group, GO terms associated with cilium movement, axoneme structure, motile cilium, sperm flagellum and microtubule motor protein complex were significantly positively enriched (NES > 0, Q value < 0.05).