Male rats emit aversive 44-kHz ultrasonic vocalizations during prolonged Pavlovian fear conditioning. Olszyński, Polowy et al.

Description

Wistar and SHR rats underwent fear conditioning (FC) with 0, 1, 6 or 10 shocks (1 s, 1.0 mA each). We have observed the rats which received 6-10 shocks, especially, to exhibit ultrasonic vocalizations (USV) which were long, flat – i.e., of constant frequency – usually within 35-50 kHz range. We named them 44-kHz USV. Here are raw data showing rats’ behavior during fear conditioning (FC) and USV playback sessions. The dataset is related to the publication in eLife by K.H. Olszynski, R. Polowy and others (DOI: 10.7554/eLife.88810), and includes four types of files: 1. Files in .xlsx format (5 folders, A–E), 2. Files in .wav format (10 recordings). 3. Files in .csv format (DBSCAN folder) 4. Files in .mat format (k-means folder) The files are accompanied by their description. 1. A. FOLDER "FC_freezing, USV (10-s-intervals)" contains data from FC: freezing and number of emitted USV, presented in 10-s intervals. B. FOLDER "FC_USV" contains data from fear conditioning: emitted USV (properties of individual USV). C. FOLDER "Playback_distance, time, USV (10-s-intervals)" contains data from playback sessions (distance traveled, time spent in the half of the cage with the speaker, heart rate, number of emitted USV and 50-kHz USV), presented in 10-s intervals, with additional analysis of USV properties emitted in response to playback. D. FOLDER "Playback_USV" contains data from playback sessions: properties of individual USV emitted in response to ultrasonic playback. E. FOLDER “Data from selected rats” contains 3 files: E.1. Step up and step down frequencies Frequencies for assessing 22-kHz-elements vs. 44-kHz-elements frequency ratio: E.2. Freezing during USV emission (data for Table 2) Percent of freezing during 22-kHz and 44-kHz USV E.3. Mean power (data for Figure 1–figure supplement 2) Mean power, frequency, and category of USV, 2. Files in .wav format. Two selected recordings, registered during fear conditioning sessions: 2.1. FC_training_Wistar_delay_6-T_#63-6. This rat is also featured in Figure 1—figure supplement 1C (top row, first from the left). 2.2. FC_training_Wistar_trace_10-T_#P44. 2.3. Recordings used for playback experiments: 1. 44-kHz long calls 2. 22-kHz long calls 3. 22-kHz short modulated calls 4. 22-kHz short flat calls 5. 50-kHz modulated calls 6. 50-kHz flat calls 7. 50-kHz trill calls 8. 50-kHz mixed calls 3. Files in .csv format (DBSCAN folder); data supporting clustering files for DBSCAN algorithm (.csv) used in Figure 3A. File names contain: experiment number (E1–3), FC (for “fear conditioning”), rat strain (W, Wistar; SHR), rat group (S, single; P, paired; T0, 0-Trial; T1, 1-Trial; T6, 6-Trial; T10, 10-Trial), rat individual number. 4. Files in .mat format (k-means folder) contain extracted call contours used for k-means algorithm (.mat) and visualized in Figure 3BC, Figure 3—figure supplement 1.

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Please see M&M section of Olszynski, Polowy et al. eLife publication; DOI: 10.7554/eLife.88810.

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