PICC catheter with Kitelock™ solution

Description

This dataset comprises biochemical and hematological laboratory values collected from critically ill patients to evaluate the effect of Kitelock catheter lock solution on laboratory markers. The aim of this study is to determine the volume of blood that needs to be drawn before laboratory analyses are no longer influenced by residual Kitelock solution. The dataset supports research on identifying potential interference in laboratory values and establishing guidelines for accurate blood sampling after catheter locking. Research Hypothesis: This study hypothesizes that residual Kitelock catheter lock solution can influence specific biochemical and hematological laboratory markers. By determining the volume of blood that needs to be discarded before accurate lab analyses can be performed, the study aims to provide evidence-based recommendations for blood sampling in clinical practice. Dataset Variables: Identification of samples: patient_ID - annonymous identifier of the patient tube_order - order of draw of tubes 1 is the first tube, 5 is the last (reference) tube tube_type - one of the 3 possible tubes (K3EDTA tube [denoted as K3EDTA]; Sodium citrate tube [denoted as Citrate]; Lithium heparin tube [denoted as LiHep]) or "exclude" if the patient was excluded from the study note - the reason for excluding the patient from the study Biochemical markers: Alkaline phosphatase (S_ALP, µkat/L) Total protein (S_PROT, g/L) Albumin (S_ALB, g/L) Urea (S_UREA, mmol/L) Creatinine (S_KREA) Sodium (S_Na, mmol/L) Potassium (S_K, mmol/L) Chloride (S_Cl, mmol/L) Calcium (S_Ca, mmol/L) Osmolality (S_OSM, mmol/kg) Magnesium (S_Mg) Qualitative hemolysis, chylomicrons, and icterus indicators (S_kHEM, S_kCHYL, S_kIKT) Hematological markers: Leukocytes (B_LEU, x 10⁹/L) Erythrocytes (B_ERY, x 10¹²/L) Hemoglobin (B_HGB, g/L) Hematocrit (B_HCT, fraction of total) Mean corpuscular volume (B_MCV, fL) Mean corpuscular hemoglobin (B_MCH, pg) Mean corpuscular hemoglobin concentration (B_MCHC, g/L) Platelets (B_TRB, x 10⁹/L) Red cell distribution width (B_RDW, fraction of total) Mean platelet volume (B_MPV, fL) Coagulation markers: Activated partial thromboplastin time (P_APTT, s) APTT ratio (P_APTR) Prothrombin time (P_PT_s, s) Prothrombin ratio (P_PT_R) International normalized ratio (P_INR) Thrombin clotting time (P_TC, s) Thrombin clotting ratio (P_TC_R) Fibrinogen (P_FB) Data Collection and Interpretation: The data were collected from routine laboratory tests conducted in an intensive care setting, with a focus on laboratory markers that can be influenced by ethylenediaminetetraacetic acid (EDTA), the main component of Kitelock catheter lock solution. By analyzing these markers, the study aims to determine the extent to which residual EDTA affects laboratory results and establish guidelines for appropriate blood sampling volumes.

Steps to reproduce

For this study, we selected 10 patients with peripherally inserted central catheters (PICC) catheters. The dead space of the catheter was approximately 1 ml and we used Kitelock solution as a locking solution for catheters. Blood samples were drawn using a closed vacuum system with three types of Vacuette (Greiner Bio-One) blood collection tubes: • K3EDTA tube: 2 ml lavender tube without separator gel, commonly used for complete blood count (CBC). • Sodium citrate tube: 2 ml blue tube without separator gel, used for coagulation studies (containing 0.109 M sodium citrate, equivalent to 3.2% w/v). • Lithium heparin tube: 2.5 ml green tube with separator gel, used for routine biochemical examinations in our hospital. Blood Draw Protocol: 1. Discard 1 ml of blood using a syringe. 2. Draw five tubes of one type (either K3EDTA, sodium citrate, or lithium heparin). The total sum of blood volumes for the tubes were as follows: o Tube 1: 3 ml (1 ml + 2 ml) o Tube 2: 5 ml o Tube 3: 7 ml o Tube 4: 9 ml o Tube 5: Used as a reference to compare results from the previous tubes. 3. Perform laboratory analyses to measure: o Basic clinical biochemistry using the Roche c702 analyzer (Roche Diagnostics, Basel, Switzerland). o Complete blood count (CBC) using the Beckman Coulter UniCell DxH 900 analyzer (Beckman Coulter, Brea, USA). o Coagulation studies using the Werfen ACL Top 750 CTS analyzer (Werfen, Bedford, USA). Results from the tubes were compared pairwise to evaluate the impact of preanalytical conditions.

Institutions

• Fakultni nemocnice Plzen

  Plzensky, Plzen
• Univerzita Karlova Lekarska fakulta v Plzni

  Plzensky, Plzen

Categories

Funders

• University Hospital Plzen

  Czechia
• Charles University

  Czechia

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