Part2_Petromurin C induces protective autophagy and apoptosis in FLT3-ITD-positive AML: synergy with gilteritinib

Published: 13 Dec 2019 | Version 1 | DOI: 10.17632/z4m2d88hgz.1
Contributor(s):

Description of this data

Treatment of acute myeloid leukemia (AML) remains inefficient due to drug resistance and relapse, particularly in patients with FMS-like tyrosine kinase 3 (FLT3)-internal tandem duplication (ITD). Marine-derived natural products have recently been used for drug development against AML. We show here that petromurin C, isolated from the culture extract of the marine-derived fungus Aspergillus candidus KUFA0062, which was isolated from the marine sponge Epipolasis sp., induces early autophagy followed by apoptotic cell death via activation of the intrinsic cell death pathway concomitant with mitochondrial stress and downregulation of Mcl-1 in FLT3-ITD mutated MV4-11 cells. Moreover, petromurin C synergized with the clinically-used FLT3 inhibitor gilteritinib at sub-toxic concentrations. Altogether our results suggest that petromurin C could act as an anti-leukemic agent alone or in combination with gilteritinib.

Experiment data files

  • Figure 3
    Cite
    • A_Hoechst and PI of MV4-11
      Cite
      • MV4-11_eto_4h
        Cite
        • MV4-11_eto(4h)
          Cite
          • 3rd
            Cite
      • Representatives
        Cite
        • 24h
          Cite
        • 48h
          Cite
        • 72h
          Cite
        • eto_4h
          Cite
    • B_Hoechst and PI of in U937
      Cite
      • Representative pictures
        Cite
        • 24h
          Cite
        • 48h
          Cite
        • 72h
          Cite
        • eto_4h
          Cite
      • U937_24h
        Cite
        • U937 with and without z-vad_24h_1st
          Cite
          • U937+zvad_24h_1st
            Cite
            • 0+zvad_24h_1st
              Cite
            • 1+zvad_24h_1st
              Cite
            • 10+zvad_24h_1st
              Cite
            • 30+zvad_24h_1st
              Cite
            • 5+zvad_24h_1st
              Cite
            • 50+zvad_24h_1st
              Cite
          • U937_24h_1st
            Cite
            • 0_24h_1st
              Cite
            • 10_24h_1st
              Cite
            • 1_24h_1st
              Cite
            • 30_24h_1st
              Cite
            • 50_24h_1st
              Cite
            • 5_24h_1st
              Cite
        • U937 with and without z-vad_24h_2nd
          Cite
          • U937+zvad_24h_2nd
            Cite
            • 0+zvad_24h_2nd
              Cite
            • 1+zvad_24h_2nd
              Cite
            • 10+zvad_24h_2nd
              Cite
            • 30+zvad_24h_2nd
              Cite
            • 5+zvad_24h_2nd
              Cite
            • 50+zvad_24h_2nd
              Cite
          • U937_24h_2nd
            Cite
            • 0_24h_2nd
              Cite
            • 10_24h_2nd
              Cite
            • 1_24h_2nd
              Cite
            • 30_24h_2nd
              Cite
            • 50_24h_2nd
              Cite
            • 5_24h_2nd
              Cite
        • U937 with and without z-vad_24h_3rd
          Cite
          • U937+zvad_24h_3rd
            Cite
            • 0+zvad_24h_3rd
              Cite
            • 1+zvad_24h_3rd
              Cite
            • 10+zvad_24h_3rd
              Cite
            • 30+zvad_24h_3rd
              Cite
            • 5+zvad_24h_3rd
              Cite
            • 50+zvad_24h_3rd
              Cite
          • U937_24h_3rd
            Cite
            • 0_24h_3rd
              Cite
            • 10_24h_3rd
              Cite
            • 1_24h_3rd
              Cite
            • 30_24h_3rd
              Cite
            • 50_24h_3rd
              Cite
            • 5_24h_3rd
              Cite
      • U937_48h
        Cite
        • U937 with and without z-vad_48h_1st
          Cite
          • U937+zvad_48h_1st
            Cite
            • 0+zvad_48h_1st
              Cite
            • 1+zvad_48h_1st
              Cite
            • 10+zvad_48h_1st
              Cite
            • 30+zvad_48h_1st
              Cite
            • 5+zvad_48h_1st
              Cite
            • 50+zvad_48h_1st
              Cite
          • U937_48h_1st
            Cite
            • 0_48h_1st
              Cite
            • 10_48h_1st
              Cite
            • 1_48h_1st
              Cite
            • 30_48h_1st
              Cite
            • 50_48h_1st
              Cite
            • 5_48h_1st
              Cite
        • U937 with and without z-vad_48h_2nd
          Cite
          • U937+zvad_48h_2nd
            Cite
            • 0+zvad_48h_2nd
              Cite
            • 1+zvad_48h_2nd
              Cite
            • 10+zvad_48h_2nd
              Cite
            • 30+zvad_48h_2nd
              Cite
            • 5+zvad_48h_2nd
              Cite
            • 50+zvad_48h_2nd
              Cite
          • U937_48h_2nd
            Cite
            • 0_48h_2nd
              Cite
            • 10_48h_2nd
              Cite
            • 1_48h_2nd
              Cite
            • 30_48h_2nd
              Cite
            • 50_48h_2nd
              Cite
            • 5_48h_2nd
              Cite
        • U937 with and without z-vad_48h_3rd
          Cite
          • U937+zvad_48h_3rd
            Cite
            • 0+zvad_48h_3rd
              Cite
            • 1+zvad_48h_3rd
              Cite
            • 10+zvad_48h_3rd
              Cite
            • 30+zvad_48h_3rd
              Cite
            • 5+zvad_48h_3rd
              Cite
            • 50+zvad_48h_3rd
              Cite
          • U937_48h_3rd
            Cite
            • 0_48h_3rd
              Cite
            • 10_48h_3rd
              Cite
            • 1_48h_3rd
              Cite
            • 30_48h_3rd
              Cite
            • 50_48h_3rd
              Cite
            • 5_48h_3rd
              Cite
      • U937_72h
        Cite
        • U937 with and without z-vad_72h_1st
          Cite
          • U937_72h
            Cite
            • 0_72h
              Cite
            • 10_72h
              Cite
            • 1_72h
              Cite
            • 30_72h
              Cite
            • 50_72h
              Cite
            • 5_72h
              Cite
          • U937_zvad_72h
            Cite
            • 0+zvad_72h
              Cite
            • 1+zvad_72h
              Cite
            • 10+zvad_72h
              Cite
            • 30+zvad_72h
              Cite
            • 5+zvad_72h
              Cite
            • 50+zvad_72h
              Cite
        • U937 with and without z-vad_72h_2nd
          Cite
          • U937+zvad_72h_2nd
            Cite
            • 0+zvad_72_2nd
              Cite
            • 1+zvad_72_2nd
              Cite
            • 10+zvad_72_2nd
              Cite
            • 30+zvad_72_2nd
              Cite
            • 5+zvad_72_2nd
              Cite
            • 50+zvad_72_2nd
              Cite
          • U937_72h_2nd
            Cite
            • 0_72h_2nd
              Cite
            • 10_72h_2nd
              Cite
            • 1_72h_2nd
              Cite
            • 30_72h_2nd
              Cite
            • 50_72h_2nd
              Cite
            • 5_72h_2nd
              Cite
        • U937 with and without z-vad_72h_3rd
          Cite
          • U937+zvad_72h_3rd
            Cite
            • 0+zvad_72_3rd
              Cite
            • 1+zvad_72_3rd
              Cite
            • 10+zvad_72_3rd
              Cite
            • 30+zvad_72_3rd
              Cite
            • 5+zvad_72_3rd
              Cite
            • 50+zvad_72_3rd
              Cite
          • U937_72h_3rd
            Cite
            • 0_72_3rd
              Cite
            • 10_72_3rd
              Cite
            • 1_72_3rd
              Cite
            • 30_72_3rd
              Cite
            • 50_72_3rd
              Cite
            • 5_72_3rd
              Cite
      • U937_eto_4h
        Cite
        • U937_eto+zvad_4h
          Cite
          • 1st
            Cite
          • 2nd
            Cite
          • 3rd
            Cite
        • U937_eto_4h
          Cite
          • 1st
            Cite
          • 2nd
            Cite
          • 3rd
            Cite
    • C_Caspase 3,7 assay in MV4-11 and U937
      Cite
  • Figure 4
    Cite
    • A_Western blots of petromurin C_Bcl family
      Cite
      • 1st
        Cite
        • MV4-11_Bcl-2_1st
          Cite
        • MV4-11_Bcl-xL_1st
          Cite
        • MV4-11_beta actin_1st
          Cite
        • MV4-11_Mcl-1_1st
          Cite
      • 2nd
        Cite
        • MV4-11_Bcl-2_2nd
          Cite
        • MV4-11_Bcl-xL_2nd
          Cite
        • MV4-11_beta actin_2nd
          Cite
        • MV4-11_Mcl-1_2nd
          Cite
      • 3rd
        Cite
        • MV4-11_Bcl-2_3rd
          Cite
        • MV4-11_Bcl-xL_3rd
          Cite
        • MV4-11_beta actin_3rd
          Cite
        • MV4-11_Mcl-1_3rd
          Cite
      • Representative pictures
        Cite
        • Bcl-2, Mcl-1
          Cite
          • Bcl-2
            Cite
          • Beta actin
            Cite
          • Mcl-1
            Cite
        • Bcl-xL
          Cite
          • Bcl-xL
            Cite
          • Beta actin
            Cite
    • B_Western blots of petromurin C_caspases and PARP-1
      Cite
      • 1st
        Cite
        • Beta actin for Caspases
          Cite
        • Beta actin for PARP-1
          Cite
        • Caspase 3
          Cite
        • Caspase 7
          Cite
        • Caspase 8
          Cite
        • Caspase 9
          Cite
        • PARP-1
          Cite
      • 2nd
        Cite
        • beta actin for Caspases 3,7
          Cite
        • Beta actin for caspases 8,9
          Cite
        • beta actin for PARP-1
          Cite
        • Caspase 3
          Cite
        • Caspase 7
          Cite
        • Caspase 8
          Cite
        • Caspase 9
          Cite
        • PARP-1
          Cite
      • 3rd
        Cite
        • Beta actin for caspases 3,7
          Cite
        • Beta actin for caspases 8,9
          Cite
        • Beta actin for PARP-1
          Cite
        • Caspase 3
          Cite
        • Caspase 7
          Cite
        • Caspase 8
          Cite
        • Caspase 9
          Cite
        • PARP-1
          Cite
      • Representative pictures
        Cite
        • beta actin for caspase 8
          Cite
        • beta actin for caspase 9
          Cite
        • beta actin for caspases 3,7
          Cite
        • beta actin for PARP
          Cite
        • caspase 3
          Cite
        • caspase 7
          Cite
        • caspase 8
          Cite
        • caspase 9
          Cite
        • PARP
          Cite

Latest version

  • Version 1

    2019-12-13

    Published: 2019-12-13

    DOI: 10.17632/z4m2d88hgz.1

    Cite this dataset

    Ha, You Na (2019), “Part2_Petromurin C induces protective autophagy and apoptosis in FLT3-ITD-positive AML: synergy with gilteritinib”, Mendeley Data, v1 http://dx.doi.org/10.17632/z4m2d88hgz.1

Statistics

Views: 4783
Downloads: 0

Categories

Health Sciences

Licence

CC BY 4.0 Learn more

The files associated with this dataset are licensed under a Creative Commons Attribution 4.0 International licence.

What does this mean?
You can share, copy and modify this dataset so long as you give appropriate credit, provide a link to the CC BY license, and indicate if changes were made, but you may not do so in a way that suggests the rights holder has endorsed you or your use of the dataset. Note that further permission may be required for any content within the dataset that is identified as belonging to a third party.

Report