Lacticaseibacillus rhamnosus MDC 2012 whole-genome

Description

Genetic and Biochemical Characterization of Lacticaseibacillus rhamnosus MDC 2012 and Its Potential in Processing of Raw Pork Meat

Steps to reproduce

Whole-genome sequencing was performed following the recommendations of Oxford Nanopore Technologies (UK), using a Flongle R10.4.1 flow cell and the ligation sequencing kit SQK-LSK114, according to the protocol specified in the g-dna-by-ligation-sqk-lsk114-document (document: Flongle-en-GDE_9161_v114_revY_30Jan2025.pdf). High-molecular-weight, high-purity DNA was isolated using a classic phenol-chloroform extraction method. The raw sequencing data were processed using the Oxford Nanopore Technologies EPI2ME (EPI2ME Desktop V5.2.3) Bacterial Genomes workflow.

Institutions

• Armbiotechnology Scientific and Production Center

Categories

Funders

• Ministry of Higher Education Science, and Technology

  Slovenia

  Grant ID: Research Project № 21AG-2I090

Licence