Standard Operating Procedures for an in vitro MMR assay
Published: 27 September 2019| Version 1 | DOI: 10.17632/z8z3yvkv9g.1
Contributors:
Maribel González-Acosta, Inga Hinrichsen, Anna Fernández, Conxi Lázaro, Marta Pineda, Guido Plotz, Gabriel CapellaDescription
Standard Operating Procedures (SOP) for nuclear proteins extraction, HEK293T cells transfection, whole cell protein extraction, mismatched plasmid substrate generation and in vitro MMR assay. Protocols were optimized and validated in González-Acosta et al 2019 (title of the manuscript: Validation of an in vitro mismatch repair assay used in the functional characterization of mismatch repair variants).
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Institutions
Institut d'Investigacio Biomedica de Bellvitge, Institut Catala d' Oncologia
Categories
Molecular Biology, Mismatch DNA Repair, Lynch Syndrome, Functional Analysis