Raw data for anticancer activity of Ormocarpum cochinchinense silver nanoparticles against PA-1 ovarian cancer cell line
Description
This dataset contains raw experimental data from the comparative evaluation of anticancer activity of silver nanoparticles and methanolic extract of Ormocarpum cochinchinense on PA-1 ovarian cancer cell line, including Phytochemical Analysis, MTT assay results, UV-Vis, FTIR, XRD, SEM, and EDAX characterization data.
Files
Steps to reproduce
Step 1: Prepare methanolic extract of Ormocarpum cochinchinense leaves (25 g leaf powder in 100 mL methanol, incubated for 24 hours at room temperature, filtered through Whatman No. 1 filter paper). Step 2: Synthesize silver nanoparticles by adding 10 mL of plant extract to 90 mL of 1 mM silver nitrate solution. Incubate in dark at room temperature for 24 hours. Centrifuge at 5000 rpm for 30 minutes, collect pellet, and redisperse in deionized water. Step 3: Characterize AgNPs using UV-Vis spectroscopy (absorbance at 420 nm), FTIR (400-4500 cm⁻¹), XRD (scan range 8.95° to 115.70°), SEM, and EDAX. Step 4: Culture PA-1 ovarian cancer cells in DMEM with 10% FBS and 1% penicillin-streptomycin at 37°C in 5% CO₂. Step 5: Treat cells with varying concentrations (7.8 to 1000 μg/mL) of plant extract and AgNPs for 24 hours. Step 6: Perform MTT assay by adding 100 μL of 0.5% MTT (5 mg/mL) to each well, incubate for 4 hours, dissolve formazan crystals in 1 mL DMSO, and measure absorbance at 570 nm. Step 7: Calculate cell viability percentage and determine IC50 values using graphical analysis.
Institutions
- Sathyabama Institute of Science and TechnologyTamil Nadu, Chennai