Global cis-acting element (cres) editing confers Bacillus pumilus LG3145 plant-beneficial functions.Bi Meiying et al.

Published: 17 July 2023| Version 1 | DOI: 10.17632/zd6x83rnpv.1
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Description

In this project, we conferred dual-plant-benefit properties to a wild-type B. pumilus, which was isolated from intensively cultivated soil, by perturbation of its genome-scale cre-like sequences. This dataset contains sixteen spreadsheets including transcriptomic profiling of 3505 genes and metabolomic data assayed using ultra performance liquid chromatography-mass spectrometry (UPLC-MS) revealed that the mutations of cre-like sequences in LG3145 relieved the CCR effect for most genes, allowing the bacterium to convert rhizodeposits,such as FAs produced by plants, into secondary metabolites, such as pigments, PG, surfactant, bacilysin, and myxochelin B through the pentose phosphate and amino acid metabolism pathways.

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Institutions

Tianjin University of Technology

Categories

Omics, Bacillus, CRISPR/Cas9

Funding

National Natural Science Foundation of China

42077212

Tianjin Technical Expert Project

14JCTPJC00479

Training Project of Innovation Team of Colleges and Universities in Tianjin, China

202210060077

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