Simultaneous quantification of curcuminoids and monoammonium glycyrrhizinate by UV-vis spectrophotomeric method
Description
This dataset contains the experimental data obtained during the validation of a UV–Vis spectrophotometric method for the simultaneous quantification of curcuminoids (CUR) and monoammonium glycyrrhizinate (MAG) in ethanol–water (1:1, v/v) at 252 nm (λmax for MAG) and 430 nm (λmax for CUR). The dataset includes specificity, calibration curves, linearity, precision, accuracy, and limits of detection and quantification, evaluated according to the Analytical Methods Validation guideline of the Colegio Nacional de Químicos Farmacéuticos Biólogos México. MAG and CUR concentrations can be simultaneously calculated using the system of equations based on absorbance additivity described in this dataset. Method accuracy was further evaluated using a series of MAG–CUR mixtures at different concentration ratios.
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Steps to reproduce
Prepare CUR and MAG standard solutions in ethanol–water (1:1, v/v) at the concentrations indicated in the calibration curves, in triplicate. Measure the absorbance of the solutions by UV–Vis spectrophotometry at 252 and 430 nm to construct individual calibration curves. A Hitachi U-5100 spectrophotometer and a quartz cuvette with a 1 cm path length were used. Determine the linear regression equations, coefficient of determination (R²), relative standard deviation (%RSD), and 95% confidence interval (95% CI) for the slope. Evaluate accuracy on a different day by preparing CUR and MAG samples at selected concentration levels within the calibration range. MAG and CUR concentrations can be simultaneously calculated using the system of equations based on absorbance additivity described in this dataset. Analyze each concentration level in triplicate. Calculate recovery (%) by dividing the experimentally determined concentration by the expected concentration and multiplying by 100.