Localization of mEmerald-EXT1 in ER tubules and sheets

Published: 28-01-2021| Version 1 | DOI: 10.17632/zdvdghgdjp.1
Contributor:
Jean-Claude Twizere

Description

Cos7 cells transfected with mEmerald-EXT1 were analyzed by Structured Illumination Microscopy (SIM) super-resolution. _SIR_PRJ.tif 3D reconstruction shows the localisation of mEmerald-EXT1 in ER tubules, while "_025.tif" shows the localization in ER sheets.

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Steps to reproduce

-Transfect Cos7 cells with on coverslips in a 6-well plate with 1 microG of mEmerald-EXT1 -24H post-transfection, fix cells with 4% paraformaldehyde in PBS for 15 min at RT. -image cells using a DeltaVision OMX SR V4 microscope using a 60x/1.42 Olympus Plan Apo N SIM objective and sCMOS cameras (Applied Precision), 2560 x 2160 pixel resolution. The SIM image reconstruction was performed with DeltaVision softWoRf 7.0 software