Raw and Processed Data for FlyPlate-BCA Drosophila Feeding Assay

Published: 23 May 2025| Version 1 | DOI: 10.17632/zf6n73z86x.1
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Description

Full raw and processed datasets (intake values, standard-curve readings, power-analysis inputs)

Files

Steps to reproduce

Data Collection: 1、Perform Drosophila feeding assays using the FlyPlate-BCA system. 2、Use the FlyPlate-BCA setup as described in the manuscript's STAR★Methods. Data Processing: 1、Extract raw feeding data from each well using the FlyIntakeAnalyzer Python package. 2、Perform calibration using BCA protein standards and calculate food intake using the provided scripts. Statistical Analysis: 1、Perform power analysis using G*Power v3.1. 2、Use GraphPad Prism v9 for statistical comparisons (t-tests, ANOVA). Data Visualization: 1、Plot cumulative intake and feeding patterns using GraphPad Prism. 2、Compare FlyPlate-BCA and CAFE results to assess sensitivity and resolving power. Reproducibility: 1、Ensure environmental consistency (25 ± 0.5 °C, 60 ± 5% RH) during experiments. 2、Maintain uniform handling practices for both FlyPlate-BCA and CAFE assays.

Institutions

Huazhong Agriculture University

Categories

Drosophila, Assessment of Food Intake Behavior, Methods Development, Biomedical Research

Related Links

Software
https://github.com/Huai-Zheng/FlyIntakeAnalyzer
is related to this dataset

Licence