Untargeted Salivary Metabolomic Profiling Uncovers Metabolic Dysregulation in Oral Squamous Cell Carcinoma Patients
Description
Raw mass spectrometry data of saliva samples from human subjects across three groups - healthy controls, oral potentially malignant disorders (OPMD), and oral squamous cell carcinoma (OSCC). Untargeted metabolomic profiling was carried out by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) on Agilent 6545 Q-TOF for the manuscript entitled "Untargeted Salivary Metabolomic Profiling Uncovers Metabolic Dysregulation in Oral Squamous Cell Carcinoma Patients”.
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Steps to reproduce
Stored saliva samples were thawed at room temperature and centrifuged at 9,000×g for 5 min to remove debris, suspended matter and mucin. The clear supernatant was transferred to a fresh tube. A 300 µL aliquot was combined with 900 µL of chilled methanol in a 1:3 ratio (saliva:methanol) to precipitate proteins. The mixture was vortexed vigorously for 2-3 min, briefly sonicated for 1-2 min to aid metabolite extraction, and left to incubate overnight for complete protein precipitation. Samples were then vortexed again and centrifuged at 17,500×g for 15 min to pellet the precipitated proteins. The metabolite-rich supernatant was dried under a stream of nitrogen, reconstituted in deionised water:methanol (1:2), and following reconstitution, the samples were injected at a volume of 5 µL for acquisition of the raw mass spectrometry data. Rest of the analysis steps are mentioned in detail in the manuscript.
Institutions
- All India Institute of Medical Sciences BhopalMadhya Pradesh, Bhopal
- Indian Institute of Science Education and Research, BhopalMadhya Pradesh, Bhopal