Sanger sequences for “Recent discovery of Amaranthus palmeri in Italy: characterization of ALS-resistant populations and sensitivity to alternative herbicides”

Published: 19 April 2021| Version 1 | DOI: 10.17632/zjwvrbtbyt.1
Contributors:
Andrea Milani,
,
,

Description

This repository contains all the electropherograms (ABI format chromatogram file, ab1) obtained by Sanger sequencing carried out for the paper entitled “Recent discovery of Amaranthus palmeri (Amaranthaceae) in Italy: characterization of ALS-resistant populations and sensitivity to alternative herbicides”. Three A. palmeri (Palmer Amaranth) populations were found in soybean fields in Italy, were ALS-resistance was suspected. Bioassay test revealed that all the three populations were cross-resistant to thifensulfuron-methyl and imazamox, due to a point mutation at position 574 of ALS. DNA of 15 plants per population, survived to the field dose of thifensulfuron-methyl, was extracted and primers AMA-2F (5’-TCCCGGTTAAAATCATGCTC-3’ / AMA-2R (5’-CTTCTTCCATCACCCTCTGT-3’) were used to amplify the region surrounding the position 574. Amplicons were purified and primers AMA-2R was used for Sanger sequencing. In order to facilitate data interpretation, the sequences have been renamed as: Population-code_plant#_CODONS CODONS might be W for tryptophan (wild type) and L for leucine (mutated). WL refers to heterozygous genotypes. Population codes are the same used in the paper. Sequences should be reverse complemented.

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Institutions

Istituto per la Protezione Sostenibile delle Piante Consiglio Nazionale delle Ricerche

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Sanger Sequencing

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