Raw data on amniotic fluid proteomics in rats
Description
These data are related to a study carried out to describe the protein changes in the amniotic fluid of rats submitted to the subchronic effects of paraben. 24 rats were randomly divided into 4 groups (n = 6/group) in control (corn oil as administration vehicle), paraben 10 (10 mg/kg), paraben 100 (100 mg/kg ) and paraben 200 (200 mg/kg). Rats were treated daily with propyl (propyl 4-hydroxybenzoate) and butylparaben (butyl 4-hydroxybenzoate). After 90 days of treatment, the animals were euthanized, and the amniotic fluid was collected and prepared for proteomics (ESI Q-TOF MS/MS).
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Steps to reproduce
We used the UniprotKB databases (www.UniProt.org.br) and the PANTHER classification system (www.pantherdb.org) to analyze the terms of gene ontology, and the Venn diagram was obtained from the Bioinformatics & Systems Biology web tool. Data were statistically analyzed using the MetaboAnalyst software (https://www.metaboanalyst.ca/) for univariate and multivariate analyses. The exponentially modified protein abundance index (emPAI) obtained by the Mascot Distiller software was used to calculate the principal component analysis (PCA). Partial least squares discriminant analysis (PLS-DA) was used to indicate the importance of proteins by variable mean importance in the projection (VIP score, α ≥ 1). A heat map was also used to compare protein abundances between groups.
Institutions
- Universidade Estadual Paulista Julio de Mesquita Filho Faculdade de Medicina Veterinaria e Zootecnia Campus de Botucatu