Journal of Invertebrate Pathology

Our study aimed to investigate the protective effect confered by immune priming in A. vulgare against S. enterica by determining its setting up and duration. We also analysed haemocyte variations in order to linked them with a potential protection against the bacterial infection. For these purposes, we conducted two distinct experiments with several independant experimental replicates: The first consisted to primed (i.e "vaccinated") individuals either with a low dose of living S. enterica bacteria, with LB broth or without injection (control). 1 day , 7 days or 15 days later, we sampled haemolymph of individuals and examined haemocyte concentrations and viabilities. Data of these experiments are available in the file "Prigot_DataHaemocytes" in the open access repository Mendeley Data. The second experiment was based on the same priming treatment than in the first (with low dose of living bacteria, LB broth or nothing). But 1 day, 7 days or 15 days later, we injected a LD50 of bacteria in all individuals and monitored their survival rates for 7 days. Datas of these experiments are available in the file "Prigot_DataSurvivals" in the open access repository Mendeley Data. Differents animals were use for the two experiments. You can also find enclosed a .txt file (Prigot_DataLegends) giving all legends of the datasets colomn heads. Datasets are ready to be used in R program for statistical analysis, see enclosed the R-script file. For any problems in the dataset comprehension, do not hesitate to contact us.

In this study, twenty live specimens of the signal crayfish (Pacifastacus leniusculus Dana) from Lake Tahoe, California, were used to obtain fresh isolates of A. astaci. As a result, 35 axenic cultures of A. astaci were obtained, as well as isolates of two apparently undescribed Aphanomyces sp. lineages. The obtained A. astaci isolates were 100 % identical to the isolates of A. astaci B-haplogroup commonly detected in Europe, and infection experiments confirmed their high virulence towards noble crayfish. One of the undescribed Aphanomyces lineages was highly similar to Aphanomyces detected previously in crustacean zooplankton (Daphnia sp.) in Central Europe, while the other lineage was distinct, most closely related (ITS sequence similarity of 93%) to either A. astaci or Aphanomyces spp. SH-2018a isolated from Astacus astacus in Finland. Neither of the Aphanomyces spp. caused crayfish mortality under experimental conditions.

Raw data for insect consumption rate, weight and susceptibility to virus challenge.

TUNEL data