International Journal of Biological Macromolecules

inititial data for viscostiy and bioadensive properties of BSP

Supplementary Files

Figure S1 SEM images of pure CE aerogels. 2 wt% (a), 3 wt% (b), 4 wt% (c and e), 5 wt% (d and f), BET nitrogen adsorption/desorption isotherm of the CE4 aerogel (g). Figure S2The images of pure CE2 (a), CE3 (b), CE4 (c) and CE5 (d) aerogels with the loading of 0 and 100 g weight. The weight of CE aerogels were 0.0382 g, 0.0565 g, 0.0745 g and 0.0892 g, respectively. Pure CE aerogels with (e) and without (f) added NaCl, CG composite aerogels with (g) and without (h) added NaCl. Figure S3: EDX spectrum and element analysis results of the CG4.5 (a) and SCG4.5 (b); EDX mapping of the CG4.5 (c) and SCG4.5 (d). Figure S4: The illustrative morphology of the SCG aerogel (a); adsorption mechanism of MB and TC by the SCG aerogel (b). Table S1 Bulk densities and liquid adsorptions of pure CE aerogels. Table S2 BET results of some representative samples. Table S3 Deconvolution of C1s peaks of CG4.5 and SCG4.5 aerogels. Table S4 Kinetics parameters for MB adsorption. Table S5 Kinetics parameters for TC adsorption. Table S6 Parameters of the isotherms models for MB and TC adsorption on the SCG aerogels.

Figure and tables supporting the manuscript

complete genome.fas The sequence of complete genome of the Phauda flammans. We checked by manual proofreading according to its relative species. tree.fas The dateset is what we used in the tree reconstruction.

The data file is mainly associated with the manuscript titled "Transgenerational effects of paternal dietary astragalus polysaccharides on spleen immunity of broilers".

Metabolomic data

Ginsenoside Rh2 (3β-O-Glc-protopanaxadiol), a trace but an important pharmacological component of ginseng, has exhibited excellent medicinal potential. Many studies have found that the synthesis of Rh2 by UDP-glucosyltransferase (UGT) is an alternative production strategy. In this study, Yjic from B. subtilis 168 was found to synthesize ginsenoside F12 (3β,12β-Di-O-Glc-protopanaxadiol) and Rh2 at a ratio of 7:3. Yjic regioselectivity toward Rh2 synthesis was successfully improved using a semi-rational design including structure-guided alanine scanning and saturation mutations. As a result, mutant M315F was found to efficiently synthesize Rh2 (~99%) and block the further glycosylation of C12-OH. The circulation of UDPG was achieved by combining M315F with AtSuSy through a cascade reaction. Furthermore, an extraordinarily high yield of Rh2 (3.7 g/L) was attained in an aqueous solvent system with 17% DMSO (v/v) through the fed-batch feeding of PPD. This study presents the high potential for the oriented preparation of ginsenoside Rh2.

Supplementary figure 1 shows the effect of different types of media on CDA activity. The cultures grown in TSB medium exhibited the maximum CDA activity as compared to the other investigated media. Supplementary figure 2A and 2B demonstrate the effect of the different nitrogen sources and different glycerol concentrations on the CDA expression. Yeast extract and beef extract exhibited almost similar enzyme expression of about 160 U/ml, followed by peptone and tryptone. Supplementary figure 3 demonstrates the SDS-PAGE picture of the various stages of purification. The purified protein showed the size of about 29 kDa, which is similar to the expected size.

Supplementary figures and figure legends for the main text.