Biomaterials

The present dataset relates to the materials engineering and characterization for methacrylated gelatin and gellan gum blending, hydrogels formation, linear porosity fabrication and mechanical and degradation assessment. Furthermore, viability and cytotoxicity evaluations were performed for cell culture purposes. Neuronal cell cultures were performed and neuron guidance characterized. PSD95 marker was assessed as a post-synaptic marker, as well as its vertical and horizontal distribution frequency were characterized.

CA2 and PB110 Cell Lines

Reliable single unit neuron recordings from chronically implanted microelectrode arrays (MEAs) are highly valuable tools in the field of neural engineering. However, following implantation MEAs undergo a foreign body response that functionally isolates them from the brain and reduces the useful longevity of the array. We tested a novel electrodeposited platinum-iridium coating (EPIC) on penetrating recording MEAs to determine if it improved recording performance. We chronically implanted the arrays in rats and used electrophysiological and histological measurements to compare quantitatively the single unit recording performance of coated vs. uncoated electrode over a 12-week period. The coated electrodes had substantially lower impedance at 1 kHz and reduced noise, increased signal-to-noise ratio, and increased number of discernible units per electrode as compared to uncoated electrodes. Post-mortem immunohistochemistry showed some correlation between glial scarring and electrophysiological metrics, although the correlation was not sufficient to account for the distinct improvements seen with the coated electrodes. Overall, the EPIC arrays provided superior recording performance than uncoated arrays, likely due to lower electrode impedance and reduced noise. This dataset contains all impedance, unit yield, noise, amplitude, signal-to-noise ratio, and immunofluorescence data necessary to reproduce these findings.

Raw human immortalized mesenchymal stem cell (hiMSC) and human macrophage cell data from ChemoTopoChip screens included in Excel (.xlsx) format. CellProfiler (Carpenter et al. 2006) generated topographical descriptors included in Excel (.xlsx) format. hiMSC, human macrophage and illumination correction CellProfiler (Carpenter et al. 2006) pipelines included in CellPropfiler (.cppr) format.

Contribution of bone marrow-derived cells to in situ engineered tissue capsules in a rat model of chronic kidney disease. Tissue engineered blood vessels (TEBVs) hold great promise for clinical use in patients with end stage renal disease (ESRD) requiring vascular access for hemodialysis. Our group has previously developed a way to generate TEBVs in situ, by utilizing foreign body response to polymeric rods that are implanted subcutaneously (Rothuizen TC, Rotmans JI et.al, Biomaterials. 2016 Jan). In the present study, we aimed to investigate the origin of the cells in the tissue capsules (TCs) that are formed around the implanted rods. In addition, we aimed to study the effect of chronic kidney disease (CKD) on TC formation. For this purpose, we utilized a rat model of CKD, which we combined with a BM-transplantation using GFP-labeled cells. These experiments revealed that both bone-marrow derived- as well as tissue resident inflammatory cells contribute to TC formation. In addition, we show that macrophages serve as precursors of myofibroblasts in mature TCs. The presence of CKD did not significantly alter the process of TC formation, which holds the potential to support our approach for future clinical use in hemodialysis patients. The raw data files contain data on each experimental animal for immunohistochemical analysis, RNA expression and laboratory measurements (blood urine nitrogen, creatinine, %of GFP+ bone marrow derived cells) of experimental model. In addition, files include data on statistical tests used in this work such as column statistics and Mann-Whitney test. All data is presented as text documents and was transferred from GraphPad Prism 7.03 program which was used for data analysis.

The 'raw data_pzf.zip' file contains the graphs of the submitted manuscript as a form of Graphpad PRISM files, which includes the raw values of each observation as embedded spreadsheets. Other images are uncropped copies of the images in the figures of the submitted manuscript.

This dataset includes data related to 3D transfection kinetics with mRMA and pDNA. Chondrogenic and myogenic gene expression and different time points upon priming with mRNA or pDNA.

Antisense oligonucleotides (ASOs) usually contain a fully phosphorothioate (PS) backbone, which possibly interacts with many genes and proteins under intracellular conditions. G3139 is an ASO that targets Bcl-2 mRNA and induces cell apoptosis. Here, we report a kind of cytidinyl-lipid combined with a cationic lipid (DNCA/CLD, molar ration, 28:3, named mix), which might interact with oligonucleotides via H-bond formation, pi-stacking and electrostatic interaction, accompanied by low zeta potentials. The IC50 value of G3139 delivered by mix-lipid reduced from above 20 uM to 0.158 uM for MCF-7/ADR, and exhibited stronger antiproliferation upon other cancer cell lines. In addition, PS modification in the 3'-half of G3139 (especial at positions 13-16) enhanced serum stability, target specificity and anticancer activity. Also, a locked nucleic acid (LNA) gapmer G3139 (LNA-G3139) showed superior antiproliferation (78.5%) and Bcl-2 mRNA suppression effects (85.5%) at 200 nM, mainly due to its high mRNA affinity. More apoptosis-associated targets were identified, and a lower level of non-specific protein binding (HSA) determined revealed that both antisense and aptamer mechanisms might simultaneously exist. A combination of a new delivery system and chemical modifications, such as in LNA-G3139, may have potential clinical application prospects in the future.

Original Image Files

Oct4+ counts for matrix array conditions tested in Ireland et al. "Combinatorial extracellular matrix microarray identifies novel bioengineered substrates for xeno-free culture of human pluripotent stem cells"