Ultrasound in Medicine & Biology

The research data is about the population characteristics, the results of imaging tests and arthroscopy in all 97 cases.

This is original data of Von Mises strain (Peak, CV, Skew, Kurt) accroding to incidence of cerebral infarction. ROC analysis results were also provided.

This document and annexed files provide instructions for building a box to hold a 12-well Transwell cell culture plate in alignment with a single element piston transducer. The design to ensure alignment is created in Solidworks, which is then physically built by laser-cutting (printing the different sides that make up the box design) on acrylic sheets and gluing the individual parts together. The bottom section of the box serves as a water bath, and the well plate rests on the wall inserts such that the bottom of the plate is coupled to the water below. Such coupling minimizes acoustic reflections that would occur at the well plate-air interface. Acoustic absorber material can be placed on the bottom of the box to further reduce reflections. We recommend filling the box with water pre-warmed to 37C to minimize cooling of the cells during experimentation. The top of the box consists of a lid whose main purpose is to align the transducer to the center of each well and keep the distance between the transducer face and cells constant. The exact files attached here are designed for a 12-well plate with 12 mm diameter inserts (Costar 3460 Corning Transwell, Corning Inc., Kennebunk, ME, USA) and an 8 mm single element transducer (IP0102HP, Valpey Fisher, Hopkinton, MA, USA), but the design can be easily modified to accommodate well plates and transducers of various sizes. Doing so requires modifying only the lid parts of the box.

The aim of this study was to study the intra e inter-rater reliability of a new semi-automatic image analysis method for the quantification of the shape of the Doppler signal and the intratendinous vascular resistance in patellar tendinopathy.Thirty athletes (27.4 years; SD: 8.57 years) with patellar intratendinous vascularity were included in a cross-sectional study (42 tendons analysed). The intratendinous blood flow was assessed by Power Doppler and the quantification ImageJ software (v.1.50b) over manual selected ROI. Two blinded observers perform the analysis of the Doppler signal (vascular index vascular resistance) and shape descriptors (number of signals, pixel intensity, area, perimeter, major diameter, minor diameter, circularity and solidity). The intraclass correlation coefficient (ICC) was calculated also the Bland-Altman’s mean of differences (MoD) and limits of agreement (LOA) were determined. Also, small real differences (SRD) and standard error of measurement (SEM) was calculated. The intra-rater reliability was maximum for area (ICC=0.999; 95%CI=0.998-0.999) and minimum for solidity (ICC=0.782; 95%CI: 0.682 - 0.853). The MoD and the LoA were very low and the relatively SRD and SEM were below 5.3% and 2% respectively. The inter-rater reliability was maximum ICC was for area (ICC= 0.993; 95%CI=0.989-0.996) and the minimum for circularity (ICC= 0.73; 95%CI=0.611-0.817). The MoD and the LoA were low, with SRD and SEM below of 6% and 2.2%. The reliability of the proposed quantitative method to study the intratendinous Doppler signal in patellar tendon is reliability and reproducibility.