Applied Soil Ecology

The file provides the data for Microbial P, phosphatase activity, Hedley fractions and pH measured in each replicate of the in situ experiment described in the paper "Response of phosphorus dynamics to sewage sludge application in an agroecosystem in northern France" by HOUBEN et al.

Supplementary File 1: Alpha diversity metrics for each soil sample Supplementary File 2: Relative abundance of 16S rRNA features (OTUs) for each sample in the dataset rarefied to 7471 reads Supplementary File 3: Relative abundance of fungal ITS features (OTUs) for each sample in the dataset rarefied to 41,958 reads

Metadata, processed data, and analysis scripts for 'Post fire litters are richer in water soluble carbon and lead to increased microbial activity'.

Script in R.data file containing data and data analysis

Original data of this work.

Original data for APSOIL_2018_217

Data noted in the excel file is of soil bacterial and fungal abundance in a southern pine forest. The study area was a Pinus taeda (loblolly pine) forest located in eastern Texas USA (31° 06’ 32.48’’ N, 95° 09’ 59.15’’W). In brief, the experimental design consisted of two different OMR intensities (low-intensity: bole-only harvest, BO; high intensity: whole-tree harvest + forest floor removal, WT+FF) and unharvested control forest plots, with three replicates per treatment. Treatments were harvested in 1996, and replanted with containerized P. taeda seedlings at 2.5 m x 2.5 m spacing. Soil was sampled seasonally for 1 year (June 2014, September 2014, December 2014, and March 2015). At each sample date, 4 cores were taken within each replicate plot to a depth of 1 m, and separated into depth increments (0-10, 10-30, 30-60, and 60-100 cm). The four cores/plot were then pooled by depth increment and homogenized to create a single homogenous sample for each replicate plot, from which a representative subsample was removed and stored at -80 °C for molecular analyses. Microbial DNA was extracted from soil as noted in Mushinski et al. (2017). Quantitative-PCR (qPCR) targeting total bacteria and fungi was performed using primer pairs 1100F/1492R for bacteria and ITS1F/ITS5.8S for fungi . The 25 μl reaction mixture contained 13 μl SYBR green real master mix (5Prime, Gaithersburg, MD), 0.5 μl of each primer (concentration 10 mM), 1 μl DNA template, and 10 μl molecular-grade water. Each analytical run included a set of standards, negative controls, and replicated samples (n = 3) on a 96-well plate. For the 16S rRNA gene, the qPCR conditions were as follows: 95 °C for 10 min; 95 °C for 30 sec, 53 °C for 30 sec (40 cycles); 72 °C for 1 min. For the ITS region, the qPCR was run with the following conditions: 94 °C for 5 min; 94 °C for 30 sec, 57 °C for 45 sec (30 cycles); 72 °C for 1.5 min. The qPCR was performed using a Mastercycler® ep realplex thermal cycler (Eppendorf, Hamburg, Germany). Amplification efficiencies of 77-80% and 84-86% were obtained for bacteria and fungi, with r2 values > 0.96. Plasmids containing 16S and ITS inserts were used for the standard curves. References Mushinski, R.M., Gentry, T.J., Dorosky, R.J., Boutton, T.W., 2017. Forest harvest intensity and soil depth alter inorganic nitrogen pool sizes and ammonia oxidizer community composition. Soil Biol. Biochem. 112, 216-227.

The Illumina Miseq sequencing data of the three treatments mentioned in the article. 0_1, 0_2, 0_3 refer to the treatment of CF;2_1, 2_2, 2_3 refer to the treatment of OF; 1_1, 1_2, 1_3 refer to the treatment of OF_EM.

TableS1 and Table S2.

This is the raw data of the soil organic carbon (SOC), oxalate extractable iron oxides (Feo), carbon mineralization rate (CMR), specific carbon mineralization rate (SCMR), microbial biomass (MBC and MBN), metabolic quotient (qCO2), and redox potential (Eh) of a rice-wheat rotation paddy soil after each wheat and rice harvest from 2013 to 2015.