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1321 results
- Data for: High-quality, high-throughput cryo-electron microscopy data collection via beam tilt and astigmatism-free beam-image shiftScript 1. Automatic coma determination program. Data was collected using the apo-ferritin sample with a continuous carbon-film grid at -2um defocus value. It is necessary to eliminate the original astigmatism and coma in manual before measurement to get original beam tilt angles (ori_X and ori_Y). Script 2. Coma search of beam-image shift. Run script 2 and record changes of astigmatism and coma in different α and d. The changes of astigmatism obtain from the Stigmator window of serialEM and coma obtain via automatic coma determination program. Fit all the parameters to get the fitting curves and formulas which are applied to the beam-image shift data collection. Script 3. 6×6 data collection via beam-image shift. In theory, only to know α0 and r (Figure 3), we can calculate all the parameters needed for data collection. But there is a slight difference between the actual and theoretical values due to the error in grid production or other objective factors.
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- Data for: Electron cryo-tomography of vestibular hair-cell stereociliaThese are electron cry-tomograms associated with the manuscript
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- Data for: NADPH-dependent sulfite reductase flavoprotein adopts an extended conformation unique to this diflavin reductase.This is the refined X-ray crystal structure of an internal deletion of monomeric sulfite reductase flavoprotein with an internal truncation that allowed us to determine the structure of each domain in the same structure.
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- Cryo-EM structure of hAQP2 in DDM
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- Human IMPDH2 mutant - L245P, treated with GTP, ATP, IMP, and NAD+; compressed filament segment reconstruction
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- Human IMPDH2 mutant - L245P, treated with ATP, IMP, and NAD+; extended filament segment reconstruction
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- Crystal structure of Mycobacterium thermoresistibile MurE in complex with ADP and 2,6-Diaminopimelic acid
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- Dimethylated RSL-R5 in complex with cucurbit[7]uril, F432 cage assembly
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- Dimethylated RSL-R5 in complex with cucurbit[7]uril, C121 sheet assembly
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- Crystal Structure of Anti-cortisol Fab in Complex with Cortisone
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