International Immunopharmacology

This is data of the study for Clinical characteristics and prognostic risk factors of Anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV).

figures

The dataset includes all statistical analysis results for the Manuscript entitled "Isodeoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber Linn., inhibits pro-inflammatory mediators’ production through both nuclear factor-κB and activator protein-1 pathways in LPS-activated macrophages". There are curently 27 files avaliable. The type of all files is GraphPad Prism 7 XML Project (.pzfx). The data were analyzed using GraphPad Prism Version 7.0. A Student’s t-test was used when only two groups were compared. When there were more than two groups, the intergroup significance was analyzed using one-way ANOVA with Tukey post hoc analysis. Differences were considered to be significant at P < 0.05.

Figures And Tables In The Article

The figures for chemical structure identification are uploaded. Moreover, the figures for HE, TUNEL and ICH experiment have been uploaded, and the scale bar is in the lower right corner, which can be seen clearly. In addition, the figures of Western blot experiment are uploaded.

In the present study we assessed how ionization radiation affects TLR4-stimulated immune activation in lipopolysaccharide (LPS)-induced cystitis. LPS or saline was administered intravesically to female rats followed by urinary bladder irradiation (20 Gy) 24 hours later or sham treatment. Presence in the urinary bladder of inflammatory cells (mast cells, CD3+, ionized calcium-binding adapter molecule 1 (Iba-1)+, CD68+, CD40+, CD80+, CD11c+ and CD206+ cells) and expression of oxidative stress (8-OHdG), hypoxia (HIF1α) and anti-oxidative responses (NRF2, HO-1, SOD1, SOD2, catalase) were assessed 14 days later with western blot, qPCR and/or immunohistochemistry. LPS stimulation resulted in a decrease of Iba-1+ cells in the urothelium, an increase in mast cells in the submucosa and a decrease in the bladder protein expression of HO-1, while no changes in the bladder expression of 8-OHdG, NRF2, SOD1, SOD2, catalase and HIF1α were observed. Bladder irradiation inhibited the LPS-driven increase in mast cells and the decrease in Iba1+ cells. Combining LPS and radiation increased the expression of 8-OHdG and number of CD3-positive cells in the urothelium and led to a decrease in NRF2α gene expression in the urinary bladder. In conclusion, irradiation may attenuate LPS-induced immune responses in the urinary bladder but potentiates LPS-induced oxidative stress, which as a consequence may have an impact on the urinary bladder immune sensing of pathogens and danger signals.

raw extracted data from all clinical trials

western blot raw data

This is the inflammation-related data of 70 patients with CAPD in our center.

All data in this paper