Stem Cell Research

The results of real time-PCR analysis of inflammatory markers comparing control & experimental IC groups, including PPS, MSC, and MSC with PPS

Raw data from Headley et al. 2019.

This file contains the statistical analysis for the heatmaps presented in Figures 3B and S4C of the paper. Specifically, the annotation, expression, and cluster membership of each gene are presented, with each tab corresponding to a heatmap analysis for the labeled one-way ANOVA.

This record contains raw data related to the article " "Reprogramming of dermal fibroblasts from a Duchenne muscular dystrophy patient carrying a deletion of exons 45–50 into an induced pluripotent stem cell line (CCMi005-A)" Abstract Duchenne muscular dystrophy (DMD) is an X-linked syndrome that affects skeletal and cardiac muscle and is caused by mutation of the dystrophin gene. Induced pluripotent stem cells (iPSCs) were generated from dermal fibroblasts by electroporation with episomal vectors containing the reprogramming factors (OCT4, SOX2, LIN28, KLF4, and L-MYC). The donor carried an out-of-frame deletion of exons 45–50 of the dystrophin gene. The established iPSC line exhibited normal morphology, expressed pluripotency markers, had normal karyotype and possessed trilineage differentiation potential.

This record contains raw data related to the article “Generation of four human induced pluripotent stem cell lines from COVID-19 hospitalized patients with increased levels of cardiac Troponin in the acute infection phase developing or not myocarditis” ABSTRACT Coronavirus disease (COVID-19) is an infectious disease caused by SARS-CoV-2 virus, leading to mild to severe respiratory symptoms. Cardiovascular involvement is frequent and mainly manifests with myocarditis, arrhythmias, cardiac arrests, heart failure and coagulation abnormality. We generated human induced pluripotent stem cells (hiPSCs) from four COVID-19 patients, all characterized by increased levels of high-sensitivity Troponin I (hsTnI) during the infection acute phase, who developed (n = 2) or not (n = 2) severe myocarditis, as COVID-19 complication. The established hiPSCs were characterized for pluripotency and genomic stability, and constitute a useful resource for studying the mechanisms underlying the variability in COVID-19 severe cardiac manifestations.

Dataset contains: - Sanger sequencing data: 4 files (ABI format chromatogram) - 2 files (pdf) of CGH array experiments

genetic analysis related to article "Generation of a human iPSC line, FINCBi001-A, carrying a homoplasmic m.G3460A mutation in MT-ND1 associated with Leber’s Hereditary Optic Neuropathy (LHON)"

This record contains raw data related to the article: "Generation of the Becker muscular dystrophy patient derived induced pluripotent stem cell line carrying the DMD splicing mutation c.1705-8 T>C." Abstract: Becker Muscular dystrophy (BMD) is an X-linked syndrome characterized by progressive muscle weakness. BMD is generally less severe than Duchenne Muscular Dystrophy. BMD is caused by mutations in the dystrophin gene that normally give rise to the production of a truncated but partially functional dystrophin protein. We generated an induced pluripotent cell line from dermal fibroblasts of a BMD patient carrying a splice mutation in the dystrophin gene (c.1705-8 T>C). The iPSC cellline displayed the characteristic pluripotent-like morphology, expressed pluripotency markers, differentiated into cells of the three germ layers and had a normal karyotype.

The dataset includes all the data for the thesis, including flow cytometry, western data, q-pcr data, cell images.The dataset includes the establishment and characterization of patient-specific iPSC, together with the differentiation and characterization of cardiomyocytes. Western blot images shows the protein expression, q-PCR data shows the mRNA expression.

The dataset includes all the data for the thesis, including flow cytometry, western data, q-pcr data, cell images.The dataset includes the establishment and characterization of patient-specific iPSC, together with the differentiation and characterization of cardiomyocytes. Western blot images shows the protein expression, q-PCR data shows the mRNA expression.