Developmental and Comparative Immunology

All raw data of Figures in "Molecular cloning and functional analysis of TRAF6 from Yangzhou great white goose Anser anser".

XCL1 and XCR1_Raw DNA sequence data

RNase1 alleviates the Aeromonas hydrophila induced oxidative stress in blunt snout bream

This data-in-Brief file included the tissue expression data of SpToll2 (Fig.2), the immune challenge data of SpToll1 and SpToll2(Fig.3-A-G), the RNAi assay data of SpToll1 and SpToll2(Fig.6-A-F), bacteria clearance assay data of SpToll1 and SpToll2 (Fig.7) and the overexpression data of SpToll1 and SpToll2 (Fig.8). In addtion, the supplementary figures in three single word documents named Fig.S1, Fig.S2, Fig.S3 were also included.

Real time RT-PCR data, raw data with calculations

This dataset supports the main conclusions of Leeuwis et al. (2024) published in Developmental & Comparative Immunology. The file consists of four datasheets: - The first and second sheets contain haematological blood properties data and immune gene expression data, respectively, as measures/parameters for innate immune function; - The third and fourth sheets contain serum immunoglobulin (IgM) level data and IgM+ leukocyte abundance data, respectively, as measures/parameters for adaptive immune function. For any questions about this dataset, please contact the corresponding author at rhjleeuwis@mun.ca.

Abstract: High-Mobility Group (HMG) proteins are involved in different processes such as transcription, replication, DNA repair, and immune response. The role of HMG proteins in the immune response of fish has been studied mainly for HMGB1, where its expression can be induced by the stimulation of viral/bacterial PAMPs and can act as a proinflammatory mediator and as a global regulator of transcription in response to temperature. However, for BbX this role remains to be discovered. In this work, we identified the BbX of E. maclovinus and evaluated the temporal expression levels after simultaneous challenge with P. salmonis and thermal stress. Phylogenetic analysis does not significantly deviate from the expected organismal relationships suggesting orthologous relationships and that BbX was present in the common ancestor of the group. BbX mRNA expression levels were very high in the intestinal tissue of E. maclovinus (foregut, midgut, and hindgut). Nevertheless, the protein levels analyzed by WB showed the highest levels of BbX protein in the liver (constitutive expression). On the other hand, the mRNA expression levels of BbX in the liver of E. maclovinus injected with P. salmonis and subjected to thermal stress showed an increase at days 16 and 20 in all treatments applied at 12 °C and 18 °C. Meanwhile, the protein levels quantified by WB showed a statistically significant increase in the HMG-Bbx at all experimental times (4, 8, 12, 16, and 20 dpi). However, at 4 dpi the HMG-Bbx protein levels were much higher than the other days evaluated. The results suggest that BbX protein may be implicated in the response mechanism to temperature and bacterial stimulation in the foregut, midgut, hindgut, and liver, according to our findings at the level of mRNA and protein. Furthermore, our WB analysis suggests an effect of P. salmonis on the expression of this protein that can be observed in condition C+ 12 °C compared to C- 12 °C. Then, there is an effect of temperature that can be evidenced in the condition AM 18 °C and SM 18 °C, compared to AB 18 °C and SB 18 °C at 4, 8, and 12 dpi. We found not differences in the levels of this protein if the thermal stress is achieved through acclimatization or shock. More research is necessary to clarify the importance of this type of HMG in the immune response and thermal tolerance in fish.