Fish and Shellfish Immunology

MetaCyc metabolic pathways were analyzed for species composition at genus level of fish.

Tilapia- a hardy species is the second most widely cultured fish next to carps globally. In 2016, the total production of tilapia was roughly about 6.69 million tonnes and has been expected to rise to 7.3 million tonnes by the end of 2030. The most distinct candidature traits of this species includes euryphagic feeding habit, captive breeding potential, tolerance to high stocking density and improved growth performance in various aquaculture systems. However, with the emerging disease problems and lack of fish seed availability posed the development of Genetically Improved Farm Tilapia (GIFT) strain using eight different species of Tilapia under selective breeding by World Fish Centre . The expansion of aquaculture limited to land and water utilization hinders the productivity of the aquaculture particularly in Tilapia farming . To overcome these bottle necks, sustainable intensification by the adoption of advanced culture systems and technologies becomes inevitable to improve the production and productivity of the sector. One of such advanced technologies is biofloc technology, a minimal or zero water exchange technology, which allows the animals to stock at higher densities. Bioflocs are conglomerates of algae, bacteria, protozoans, fecal matter and uneaten feed which are held together in a loose matrix by the secretions of filamentous microorganisms or by electrostatic attraction

Characterization of the effects of MDA5 and LGP2 on induction of IFN-β, NF-𝜅B and IRF3. MDA5 293T cells and LGP2 293T cells were seeded in 6 well plates and treated with 10ng/ml tetracycline, respectively, after seventy-two hours culturing, the cells were stimulated with poly I:C (1mg/mL) or LPS (1mg/mL). Ten hours later, the cells were treated with RIPA to carry out the western blot analysis. Gray value detection of western blot were repeated at least three times, and rubber map of western blot was used.