Molecular Cell

Raw data Flury et al, Molecular Cell, 2017

Data for protein purification of Fig. 2F, GFP fluorescent processed full image of Fig. S2A, western blot images of Fig. S2B, S5B. For detailed description and methods refer paper.

The approximately thirty core subunits of kinetochores assemble on centromeric chromatin containing the histone H3 variant CENP-A and connect chromosomes with spindle microtubules. The chromatin proximal 16-subunit CCAN (constitutive centromere associated network) creates a mechanically stable bridge between CENP-A and the kinetochore’s microtubule-binding machinery, the 10-subunit KMN assembly. Here, we reconstituted a stoichiometric 11-subunit human CCAN core that forms when the CENP-OPQUR complex binds to a joint interface on the CENP-HIKM and CENP-LN complexes. The resulting CCAN particle is globular and connects KMN and CENP-A in a 26-sub- unit recombinant particle. The disordered, basic N-terminal tail of CENP-Q binds microtubules and promotes accurate chromosome alignment, cooperating with KMN in microtubule binding. The N-terminal basic tail of the NDC80 complex, the microtubule-binding subunit of KMN, can function- ally replace the CENP-Q tail. Our work dissects the connectivity and architecture of CCAN and re- veals unexpected functional similarities between CENP-OPQUR and the NDC80 complex.

Data for manuscript titled, "Cryo-EM Structures of Human Drosha and DGCR8 in Complex with Primary MicroRNA"

This data accompanies the paper "mTOR Inhibition Restores Amino Acid Balance in Cells Dependent on Catabolism of Extracellular Protein" published in Mol Cell, 2017.

All raw microscopy images and blots for Penn et al.

imaging data

Raw image files for the paper entitled "Molecular basis for the single-nucleotide precision of primary microRNA processing".

Online on December 6th, 2018 in : Molecular Cell Print: Molecular Cell 73, 1–14, January 17, 2019 This is the original data set that contains all of the unprocessed microscopy images, gels, and blots used in this manuscript.

Raw imaging & fluorescence data belonging to the manuscript 'Structural Mechanistic Insights into Cis- and Trans-acting deoxyribonuclease activities of Cas12a' by Swarts. Both raw image files and unprocessed .tif image files are uploaded. The .pzfx files are GraphPad Prism files used to generate graphs based on gel scans and band signal intensity with the same name. Please not that trans-activity assays do not also contain images of gels as generated fluorescence was measured directly as described in the STAR methods. For (raw) gel images used for Figure 5, please see the identical gels used for Figure S7 and S8. The excel file contains the raw Size Exclusion Chromatography absorption and fluorescence readouts. The .PDB and .mtz files corresponding to the manuscript are uploaded at the PDB database.