Dropen is a fully automatic drop image analysis software in order to study the wetting properties of solid surfaces. It also includes the manual selection and fitting approaches. This software is developed in MATLAB using GUIDE.
Regarding the identification of contact points two approaches are provided in Dropen:
1. Automatic: determines the left and right contact points automatically using a precise image processing code;
2. Manual: let the user select the left and right contact points manually by pushing the “Manual selection” button. In the case of the wrong selection of contact points the software let the user try two more times without a need to push the “Manual selection” button again.
Regarding the contact angle measurement, the software provides a fully- automatic convolution mask method and also fitting with the circle and polynomial equations. So, the contact angle measurement panel includes:
1. Mask method: the fully automatic contact angle measurement,
2. Fitting: includes:
b. Polynomial: user can change the polynomial order. Regarding our studies, the optimum polynomial order for superhydrophilic droplets is two and for the rest of the droplets is Three. So, it is recommended to do not to change the polynomial order.
After choosing the contact point identification and contact angle measurement methods, push the RUN button to find the results in the Result panel. In addition, the magnified images of the left and right contact areas are presented there. The drop contour line and the contact points have been shown with blue and red markers on the image, respectively. It is possible to save the contact angle data, as well as the left and right contact area images by pushing the corresponding push buttons.
This software is developed in SEFILAB at the Department of Materials science, University of Milano-Bicocca, and is available at the UNIMIB repository (http://dx.doi.org/10.17632/wzchzbm58p.3). This repository will be updated by the new modified versions of the software.
Contributors:Giorgio Cattoretti, Maddalena M Bolognesi, Francesca M Bosisio, Mario Faretta, Francesco Mascadri et al
Background: Antibody validation for tissue staining is required for reproducibility and criteria to ensure validity have been published recently. The majority of these recommendations imply the use of routinely processed tissue (FFPE).
Materials & Methods: We applied to lightly fixed frozen sections a panel of 126 antibodies validated for FFPE with extended criteria.
Results: Less than 30% performed conservatively with all fixations, 35% preferred one fixation over another, 13% gave non-specific staining, 23% did not stain at all.
Conclusions: Individual antibody variability of the paratope fitness for the fixed antigen may be the cause. Re-validation of established antibody panels is required when applied to sections whose fixation and processing is different from the tissue where they have been initially validated.
These are supplementary Data to the manuscript. In addition Tabel 1 and Supplementary Table 1 are provided in Excel format.