Reproductive Biology

uploaded files represent primary-cultured (P1 suffix) and long term-cultured data for semi-quantitative analysis of the expression of pluripotency-related genes in human amniotic fluid cells

We examined if leptin could alter the transcriptome of the constitutively steroidogenic rat tumor Leydig cell line R2C. These cells were treated with high levels leptin (1000 ng/ml) for 4 h, followed by mRNA extraction and RNA-Seq analysis. Total RNA extractions from cultured R2C Leydig cells were performed using the E.Z.N.A. Total RNA kit (Omega Bio-Tek, Inc., Norcross, GA). Libraries were constructed using the TruSeq Stranded mRNA LT (Illumina) for cDNA synthesis and polyA enrichment. Quality and quantification of libraries were assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies) and KAPA SYBR® FAST qPCR Kit (KAPA Bio Systems), respectively. Transcriptomic sequencing was performed on an Illumina NextSeq 500 in paired reads layout with approximately 13 million reads per sample and 75 bp of sequencing length. Libraries and next generation sequencing were completed by Applied Biological Materials Inc. (Richmond, BC, CAN). Paired-end raw reads are available in FASTQ format.

This file contains coordinates for mammalian CatSper, modeled based on the voltage-gated calcium channel from A. Butzleri (4MS2) using conserved cysteine linkages

Attached is a model for mouse CatSper1-4 as described in "Intramembranal disulfide cross-linking illucidates the super-quaternary structure of mammalian CatSpers" by Bystroff. The file can be viewed by PyMol or any molecular display program that accepts PDB format. Four tetramer models are shown comprised of two linear dimers, arranged ntiparallel and cross-linked at CatSper3. The two-row polymer is proposed to extend the length of the principle piece of the sperm flagellum.